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. 1982 Jul;37(1):172-8.
doi: 10.1128/iai.37.1.172-178.1982.

Production and partial characterization of monoclonal antibodies to Mycobacterium leprae

T P Gillis, T M Buchanan

Production and partial characterization of monoclonal antibodies to Mycobacterium leprae

T P Gillis et al. Infect Immun. 1982 Jul.

Abstract

Monoclonal antibodies to Mycobacterium leprae were produced by the fusion of BALB/c splenocytes and lymph node cells to BALB/c myeloma (NSI/1) cells. Eleven monoclonal antibodies were characterized as to their reactivity with M. leprae and 18 other mycobacterial species by enzyme-linked immunosorbent assay and immunofluorescence. Two monoclonal antibodies reacted only with M. leprae, and the other nine showed unique patterns of reactivity by enzyme-linked immunosorbent assay. One monoclonal antibody (IIH9) reacted with a 68,000-dalton protein present in extracts from M. leprae, M. tuberculosis H37Rv, M. gastri, and M. smegmatis. Potential uses for these antibodies in serological tests and immunochemical analyses are discussed.

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References

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