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. 1982 Jul;14(4):545-52.
doi: 10.1007/BF01011887.

The demonstration of B cells in pancreatic islets with orcein

F Callea, V J Desmet

The demonstration of B cells in pancreatic islets with orcein

F Callea et al. Histochem J. 1982 Jul.

Abstract

Orcein stains granules in pancreatic islet cells selectively. The localization of Orcein-positive cells within islets differs from that of Grimelius-stained cells, but corresponds to the B cell type differentiated by Aldehyde Fuchsin. Usually there appear to be fewer Orcein-positive cells than Aldehyde Fuchsin-positive ones. This indicates either that Aldehyde Fuchsin is a more sensitive stain for B cells or that Orcein is a more selective stain for a B cell type subpopulation. The rationale of the Orcein reaction in B cells seems to depend on the oxidation of disulphide bonds present in insulin and its precursors rich in cystine.

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