Automated, quantitative cytopathic effect reduction assay for interferon
- PMID: 6181091
- PMCID: PMC272375
- DOI: 10.1128/jcm.16.2.413-415.1982
Automated, quantitative cytopathic effect reduction assay for interferon
Abstract
A rapid, cytopathic effect reduction assay for human interferon (IFN) is described. Dilutions of IFN were made with an automated diluter in 96-well microtiter plates. Total incubation time was 26 h. IFN titers were calculated from optical density readings of crystal violet-stained monolayers in an automated spectrophotometer, which required less than 1 min to read each plate.
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