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. 1982 Jul 1;205(1):167-71.
doi: 10.1042/bj2050167.

Fractionation of membrane proteins on phenylboronic acid-agarose

G T Williams, A P Johnstone, P D Dean

Fractionation of membrane proteins on phenylboronic acid-agarose

G T Williams et al. Biochem J. .

Abstract

Detergent-solubilized plasma-membrane proteins from lymphocytes of patients with chronic lymphocytic leukaemia were applied to phenylboronic acid-agarose. Some of the polypeptides were bound specifically and subsequently eluted with sorbitol. Immunoprecipitation analyses showed that no surface immunoglobulin M was bound, but that most of the histocompatibility antigens HLA-A, HLA-B, HLA-C and HLA-DR were.

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