Bone marrow transplantation. Separation of stem cells

Abstract

Attempts were made to separate haemopoeitic stem cells of murine and human bone marrow by fractionating the native bone marrow on Verografin gradient, density 1.077. The in vivo and in vitro methods demonstrated that the numbers of stem cells present following fractionation on the gradient layer were significantly increased as compared to the controls. The stem cells form haemopoietic CFU-S colonies in murine spleens, as well as CFU-C during culture in agar medium. The simple, easily reproducible and routine techniques are believed to be a valuable help in practice.