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. 1982 Sep;16(3):473-7.
doi: 10.1128/jcm.16.3.473-477.1982.

Rapid diagnosis of rotavirus infection by direct detection of viral nucleic acid in silver-stained polyacrylamide gels

A J Herring, N F Inglis, C K Ojeh, D R Snodgrass, J D Menzies

Rapid diagnosis of rotavirus infection by direct detection of viral nucleic acid in silver-stained polyacrylamide gels

A J Herring et al. J Clin Microbiol. 1982 Sep.

Abstract

A rapid simple technique for the diagnosis of rotavirus has been developed based on the sensitive detection of rotavirus double-stranded RNA genome segments separated in polyacrylamide gels. The method utilizes a recently described ultrasensitive silver stain for polypeptides, which can also detect subnanogram amounts of nucleic acid. The sensitivity of the technique is comparable with that of electron microscopy or enzyme-linked immunosorbent assay.

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