Comparative Study
doi: 10.1016/0361-9230(82)90104-6.
A simplified Timm stain procedure compatible with formaldehyde fixation and routine paraffin embedding of rat brain
- PMID: 6182964
- DOI: 10.1016/0361-9230(82)90104-6
Item in Clipboard
Comparative Study
A simplified Timm stain procedure compatible with formaldehyde fixation and routine paraffin embedding of rat brain
Brain Res Bull.
1982 Jun.
Abstract
A simplified sulphide/silver technique for visualizing transitional metals in rat brain is described. It consists of: (1) sulphide perfusion before formaldehyde perfusion, (2) routine paraffin embedding and sectioning, and (3) silver staining with physical developer. This method is compatible with formaldehyde fixation, eliminates the need for post-fixation in Carnoy's solution before embedding and does not require low paraffin temperatures during embedding. Once embedded, brains can be stored for months before sectioning without loss of stain quality.
Similar articles
-
An improved Timm sulphide silver method for light and electron microscopic localization of heavy metals in biological tissues.Histochemistry. 1978 Feb 3;55(1):27-40. doi: 10.1007/BF00496691. Histochemistry. 1978. PMID: 76622
-
An indirect method for quantitation of cellular zinc content of Timm-stained cerebellar samples by energy dispersive X-ray microanalysis.Histochemistry. 1988;89(5):493-7. doi: 10.1007/BF00492607. Histochemistry. 1988. PMID: 2459086
-
Histochemical demonstration of heavy metals. A revised version of the sulphide silver method suitable for both light and electronmicroscopy.Histochemistry. 1981;71(1):1-16. doi: 10.1007/BF00592566. Histochemistry. 1981. PMID: 6785259
-
A novel non-perfusion Timm method for human brain tissue.J Neurosci Methods. 1990 Nov;35(2):125-31. doi: 10.1016/0165-0270(90)90102-l. J Neurosci Methods. 1990. PMID: 1704471
-
A perfusion-fixation procedure for the concurrent demonstration of Timm's, horseradish peroxidase (HRP), and acethycholinesterase (AChE) histochemistry.J Histochem Cytochem. 1984 Oct;32(10):1113-6. doi: 10.1177/32.10.6481150. J Histochem Cytochem. 1984. PMID: 6481150
Cited by
-
Disruption of the metallothionein-III gene in mice: analysis of brain zinc, behavior, and neuron vulnerability to metals, aging, and seizures.J Neurosci. 1997 Feb 15;17(4):1271-81. doi: 10.1523/JNEUROSCI.17-04-01271.1997. J Neurosci. 1997. PMID: 9006971 Free PMC article.
-
Distribution of ischemic neuronal damage in the dorsal hippocampus of rat.Acta Neuropathol. 1988;76(4):411-21. doi: 10.1007/BF00686979. Acta Neuropathol. 1988. PMID: 2459897
-
Metallothioneins and zinc dysregulation contribute to neurodevelopmental damage in a model of perinatal viral infection.Brain Pathol. 2006 Jan;16(1):1-14. doi: 10.1111/j.1750-3639.2006.tb00556.x. Brain Pathol. 2006. PMID: 16612977 Free PMC article.
-
cAMP response element-binding protein-mediated gene expression increases the intrinsic excitability of CA1 pyramidal neurons.J Neurosci. 2007 Dec 12;27(50):13909-18. doi: 10.1523/JNEUROSCI.3850-07.2007. J Neurosci. 2007. PMID: 18077703 Free PMC article.
-
Myoloid-related protein 8, an endogenous ligand of Toll-like receptor 4, is involved in epileptogenesis of mesial temporal lobe epilepsy via activation of the nuclear factor-κB pathway in astrocytes.Mol Neurobiol. 2014 Feb;49(1):337-51. doi: 10.1007/s12035-013-8522-7. Epub 2013 Aug 28. Mol Neurobiol. 2014. PMID: 23982744
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
