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. 1982 Oct 11;10(19):5809-22.
doi: 10.1093/nar/10.19.5809.

The molecular cloning and characterisation of cDNA coding for the alpha subunit of the acetylcholine receptor

K Sumikawa, M Houghton, J C Smith, L Bell, B M Richards, E A Barnard

The molecular cloning and characterisation of cDNA coding for the alpha subunit of the acetylcholine receptor

K Sumikawa et al. Nucleic Acids Res. .

Abstract

A rare cDNA coding for most of the alpha subunit of the Torpedo nicotinic acetylcholine receptor has been cloned into bacteria. The use of a mismatched oligonucleotide primer of reverse transcriptase facilitated the design of an efficient, specific probe for recombinant bacteria. DNA sequence analysis has enabled the elucidation of a large part of the polypeptide primary sequence which is discussed in relation to its acetylcholine binding activity and the location of receptor within the plasma membrane. When used as a radioactive probe, the cloned cDNA binds specifically to a single Torpedo mRNA species of about 2350 nucleotides in length but fails to show significant cross-hybridisation with alpha subunit mRNA extracted from cat muscle.

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