[RT1-B region associated antigens in Japanese inbred strains of rats: serological and immunochemical analyses]

Abstract

Ten different alloantisera absorbed with red blood cells (RBC) from appropriate strains of rats detected a series of B cell alloantigenic specificities by complement dependent cytotoxicity test, and that could be divided into three groups, presumably coded for by at least three different loci. The three loci were designated provisionally as Ba-1, Ba-3 and Ba-2 having two, three and six alleles, respectively, by analyzing strain distribution of Ba antigens. Each specificities were also designated Ba-1.1, 1.2, Ba-3.1, 3.2, 3.3, Ba-2.1, 2.2, 2.4, 2.9, 2.6, 2.7. The specificities from Ba-1 and Ba-3 groups were distributed in many strains of rats, and the specificities from the Ba-2 group were distributed in a limited strains of rats. By immunogenetical study, associations of the Ba specificity with RT1-Aa specificities, and of the Ba specificity with MLCR phenotype were observed. Immunochemically, Ba-1, Ba-3, Ba-2 antigenic specificities were studied using the B cell leukemia, KNL-14 cells, which was induced in the WKA rat with 1-buthy-1-nitrosourea, and it was clarified that as far as Ba-1.2, Ba-2.7, Ba-3.3 specificities were concerned, the three antigen specificities were found to be on the different rat Ia-like molecules, thus supporting a postulation of the existence of three loci in the RT1-B region, which was suggested by serological analyses.