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. 1982 Nov 25;10(22):7133-44.
doi: 10.1093/nar/10.22.7133.

Molecular cloning of seal myoglobin mRNA

D Wood, A Blanchetot, A J Jeffreys

Molecular cloning of seal myoglobin mRNA

D Wood et al. Nucleic Acids Res. .

Abstract

Grey seal skeletal muscle containing high levels of myoglobin was used to prepare poly(A)+ RNA. In vitro translation of this RNA produced a range of polypeptides including myoglobin. cDNA was prepared by reverse transcription of muscle poly(A)+ RNA and cloned into the plasmid pAT 153. 4% of cDNA recombinants were shown to contain myoglobin cDNA inserts. DNA sequence analysis of one clone (pSM 178) which contained a relatively large myoglobin cDNA insert showed an incomplete cDNA comprising the terminal 293 nucleotides of 3' non-translated mRNA sequences. Hybridization experiments using this myoglobin cDNA indicated that seal myoglobin is coded by a single gene which is transcribed to give a 1400 nucleotide mRNA considerably longer than related haemoglobin mRNAs.

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