doi: 10.1073/pnas.80.5.1221.
Antibodies specific for NADPH-binding region of enzymes possessing dehydrogenase activities
- PMID: 6187008
- PMCID: PMC393566
- DOI: 10.1073/pnas.80.5.1221
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Antibodies specific for NADPH-binding region of enzymes possessing dehydrogenase activities
Proc Natl Acad Sci U S A.
1983 Mar.
Abstract
The results reported in this paper show the presence of a population of antibodies in rabbit polyclonal antiserum that recognize an antigenic site at the NADPH-binding region of enzymes possessing dehydrogenase activities. Antisera from rabbits immunized with glucose-6-phosphate dehydrogenase or fatty acid synthetase were found to inactivate the enzyme dihydrofolate reductase. The inhibitory effect of this site-specific antibody is a time- and concentration-dependent reaction. This immunoinactivation is prevented by preincubation of the enzyme with NADPH.
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