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. 1983 Mar;80(5):1221-3.
doi: 10.1073/pnas.80.5.1221.

Antibodies specific for NADPH-binding region of enzymes possessing dehydrogenase activities

S S Katiyar, J W Porter

Antibodies specific for NADPH-binding region of enzymes possessing dehydrogenase activities

S S Katiyar et al. Proc Natl Acad Sci U S A. 1983 Mar.

Abstract

The results reported in this paper show the presence of a population of antibodies in rabbit polyclonal antiserum that recognize an antigenic site at the NADPH-binding region of enzymes possessing dehydrogenase activities. Antisera from rabbits immunized with glucose-6-phosphate dehydrogenase or fatty acid synthetase were found to inactivate the enzyme dihydrofolate reductase. The inhibitory effect of this site-specific antibody is a time- and concentration-dependent reaction. This immunoinactivation is prevented by preincubation of the enzyme with NADPH.

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