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. 1983 Feb 11;11(3):647-58.
doi: 10.1093/nar/11.3.647.

Unmethylated domains in vertebrate DNA

D N Cooper, M H Taggart, A P Bird
Free PMC article

Unmethylated domains in vertebrate DNA

D N Cooper et al. Nucleic Acids Res. .
Free PMC article

Abstract

We have detected a fraction that is rich in unmethylated HpaII and HhaI sites by end-labelling HpaII fragments of chicken DNA. The fraction is not obvious when DNA fragments are stained with ethidium bromide as it amounts to less than 2% of the genome. The average frequency of sites for HpaII is over thirteen times greater in the unmethylated fraction than in total DNA. Partial digests indicate that the unmethylated sites are clustered in the genome. Similar unmethylated fractions were detected in six other vertebrates in both somatic and germ line DNA.

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