Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1983 Jan;80(2):472-6.
doi: 10.1073/pnas.80.2.472.

Isolation and DNA sequence of a full-length cDNA clone for human X chromosome-encoded phosphoglycerate kinase

A M Michelson, A F Markham, S H Orkin
Comparative Study

Isolation and DNA sequence of a full-length cDNA clone for human X chromosome-encoded phosphoglycerate kinase

A M Michelson et al. Proc Natl Acad Sci U S A. 1983 Jan.

Abstract

Phosphoglycerate kinase (PGK), a major enzyme in glycolysis, is encoded by the X chromosome in mammals. We have initiated molecular analysis of the PGK structural gene by isolating a full-length cDNA clone from a human fetal liver cDNA library. Synthetic oligonucleotide mixtures encoding two different portions of PGK were used as direct in situ hybridization probes for the cDNA library. Several classes of clones were obtained based on their hybridization at different stringencies to one or both of the PGK oligonucleotide mixtures. One clone, designated pHPGK-7e, which hybridized at high stringency to each of the synthetic probes, encoded the complete PGK protein sequence as well as 82 base pairs of 5' and 437 base pairs of 3' untranslated regions. Southern blot analysis of human genomic DNAs revealed a complex pattern of hybridizing fragments, two of which were non-X in origin. These results suggest that the human genome contains a small family of dispersed PGK or PGK-like genes.

PubMed Disclaimer

References

    1. Nucleic Acids Res. 1982 Mar 11;10(5):1557-78 - PubMed
    1. Cell. 1980 Nov;22(2 Pt 2):361-70 - PubMed
    1. J Biol Chem. 1980 Dec 25;255(24):12073-80 - PubMed
    1. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5461-5 - PubMed
    1. Nucleic Acids Res. 1979 Aug 10;6(11):3543-57 - PubMed

Publication types

Associated data