doi: 10.1016/0006-291x(83)91394-3.
Localization of the proteinase-induced thiol groups in alpha 2-macroglobulin
- PMID: 6188466
- DOI: 10.1016/0006-291x(83)91394-3
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Localization of the proteinase-induced thiol groups in alpha 2-macroglobulin
Biochem Biophys Res Commun.
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Abstract
Free thiol groups released on proteolytic attack of alpha 2-macroglobulin by trypsin or chymotrypsin bind covalently to thiopropyl-Sepharose, indicating that they are located at the surface of the complexes. These cysteine sulfhydryl groups appear to be in contact with the alpha 2M-bound proteases from singlet-singlet energy transfer measurements between fluorescein isothiocyanate-labeled proteinases and N-(iodoacetylaminoethyl)-5-naphtylamine-1-sulfonic acid-labeled thiols in alpha 2-macroglobulin.
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