Alterations in number of protofilaments in microtubules assembled in vitro
- PMID: 618894
- PMCID: PMC2109969
- DOI: 10.1083/jcb.76.1.223
Alterations in number of protofilaments in microtubules assembled in vitro
Abstract
Tubulin from bovine brain was polymerized in vitro using a variety of assembly conditions. Many of the formed microtubules were shown to contain 14 wall protofilaments. The number of microtubules containing 14 protofilaments increased with consecutive repetitions of cold-dissociation followed by reassembly in vitro.
