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. 1983 May;80(9):2481-5.
doi: 10.1073/pnas.80.9.2481.

Location and distribution of the light chains in clathrin trimers

T Kirchhausen, S C Harrison, P Parham, F M Brodsky

Location and distribution of the light chains in clathrin trimers

T Kirchhausen et al. Proc Natl Acad Sci U S A. 1983 May.

Abstract

Mouse monoclonal antibodies have been made that are specific for one of the light chains (LC-A) of calf brain clathrin. The determinant they recognize has been mapped by immunoelectron microscopy and shown to lie near the center of a clathrin trimer. Quantitative immunoprecipitation experiments with one of the monoclonal antibodies suggests that, despite the overall 1:2 stoichiometry, the light chain composition of an individual clathrin trimer is nearly random.

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