Anatomical evidence for segregated focal groupings of efferent cells and their terminal ramifications in the cerebellothalamic pathway of the monkey

Abstract

Patterns of termination of the cerebellothalamic pathway were investigated using anterograde tracing techniques. The thalamic projections from each of the deep cerebellar nuclei are topographically organized in two and possibly in three dimensions. First, the caudo-rostral cerebellar nuclear dimension is mapped onto the mediolateral dimension within the cell-sparse ventral lateral thalamic region (VPLo, VLc, VLps, and nucleus X). By correlating this topographic ordering with the previously established lamellar organization of the cell-sparse thalamic region a somatotopy is inferred within the deep cerebellar nuclei, with caudal body parts represented anteriorly and rostral body parts represented posteriorly in each nucleus. A second topography consists of the mapping of the mediolateral dimension of the dentate and interpositus nuclei onto the ventrodorsal dimension of the lamellae in the thalamus. Since the thalamic connections with motor cortex predict a somatotopic organization with distal body parts ventral and axial parts dorsal in thalamus, each cerebellar nucleus should, therefore, represent axial body parts laterally and distal parts medially. A third mapping dimension is shown for the dentatothalamic projection: dorsal parts of the dentate nucleus project posteriorly within the cell-sparse thalamic region, and ventral parts project anteriorly. The significance of this as regards representation of the body is not known. Subsidiary foci of terminations within the cell-sparse thalamic region are visible following tritiated amino acid injections into each of the deep cerebellar nuclei. Following dentate injections these foci appear as anteroposteriorly elongated, rod-like aggregations of terminations which are similar to the rod-like aggregations of thalamocortical relay cells which have been demonstrated following focal injections of horseradish peroxidase into the motor cortex. The interpositothalamic and the fastigiothalamic terminations are elongated and appear as focal clusters in all planes of section. The interpositothalamic clusters are distributed within posterodorsally curving planar sheets. An anterograde double labeling technique, using a combination of the autoradiographic technique with the axonal degeneration technique, was used to investigate the interrelations of the terminations from different nuclei and from different parts of the same nucleus. Rods from different parts of the dentate nucleus terminate independently of one another. Dentatothalamic rods and interpositothalamic clusters, though interdigitating within the same thalamic region, do not overlap. This topographic and modular organization of the cerebellothalamic pathway suggests that the cerebellar input may reflect both the somatotopic and the columnar organization of the motor cortex.