doi: 10.1016/0014-5793(83)80256-7.
Titration of the binding sites for the oligomycin-sensitivity conferring protein in beef heart submitochondrial particles
- PMID: 6189744
- DOI: 10.1016/0014-5793(83)80256-7
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Titration of the binding sites for the oligomycin-sensitivity conferring protein in beef heart submitochondrial particles
FEBS Lett.
.
Free article
Abstract
The binding parameters of the oligomycin-sensitivity conferring protein (OSCP) in inside-out particles from beef heart mitochondria have been tested by means of two assays, the oligomycin-sensitive ATP-Pi exchange, and the oligomycin-sensitive ATP hydrolysis. The total number of OSCP binding sites in A particles was equal to 220 pmol/mg particle protein. Each mole of ATPase active site was able to bind 1.1 +/- 0.5 mol OSCP with Kd 1.7 nM.
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