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. 1983 Jul;31(7):938-44.
doi: 10.1177/31.7.6189883.

Immunogold-silver staining: new method of immunostaining with enhanced sensitivity

C S Holgate, P Jackson, P N Cowen, C C Bird

Immunogold-silver staining: new method of immunostaining with enhanced sensitivity

C S Holgate et al. J Histochem Cytochem. 1983 Jul.

Abstract

A new method for demonstrating antigens in paraffin sections of formol sublimate-fixed tissue is described that utilizes an "indirect" immunohistological technique employing immunoglobulin adsorbed to colloidal gold as the secondary antiserum. The gold particles introduced to antigenic sites are revealed by a silver precipitation reaction. This technique, the immunogold-silver staining method, is of much enhanced sensitivity (up to 200-fold) as compared with standard immunoperoxidase and immunogold staining methods. The results have been confirmed in a study of immunoglobulins in reactive human tonsil. The use of this new method for double immunolabeling is also described.

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