doi: 10.1016/0014-5793(83)81123-5.
NMR analyses on the molecular mechanism of the conformational rigidity of 2-thioribothymidine, a modified nucleoside in extreme thermophile tRNAs
- PMID: 6190675
- DOI: 10.1016/0014-5793(83)81123-5
Item in Clipboard
NMR analyses on the molecular mechanism of the conformational rigidity of 2-thioribothymidine, a modified nucleoside in extreme thermophile tRNAs
FEBS Lett.
.
Free article
Abstract
1H-NMR analyses have been made on the conformations of 2-thioribothymidine (s2T), 2-thiodeoxyribothymidine (s2dT), as well as ribothymidine (T) and deoxyribothymidine (dT). s2T and s2dT exclusively take the anti form rather than the syn form. The C3'-endo-gg form of the sugar moiety is remarkably stabilized on modification of T to s2T, but not on modification of dT to s2dT. The steric effects of the 2-thiocarbonyl group and the 2'-hydroxyl group cause the rigidity of the C3'-endo-gg form of s2T. Such rigidity of s2T probably contributes to the thermostability of 2-thiopyrimidine polyribonucleotides and extreme thermophile tRNAs.
Similar articles
-
Separation and comparison of 2-thioribothymidine-containing transfer ribonucleic acid and the ribothymidine-containing counterpart from cells of Thermus thermophilus HB 8.Biochemistry. 1983 Jan 4;22(1):98-102. doi: 10.1021/bi00270a014. Biochemistry. 1983. PMID: 6830766
-
Selective utilization of 2-thioribothymidine- and ribothymidine-containing tRNAs by the protein synthetic systems of Thermus thermophilus HB 8 depending on the environmental temperature.J Biochem. 1984 Nov;96(5):1625-32. doi: 10.1093/oxfordjournals.jbchem.a134993. J Biochem. 1984. PMID: 6084662
-
Dynamic structures and functions of transfer ribonucleic acids from extreme thermophiles.Adv Biophys. 1987;23:115-47. doi: 10.1016/0065-227x(87)90006-2. Adv Biophys. 1987. PMID: 2449804 Review.
-
CD and NMR studies on the conformational thermostability of 2-thioribothymidine found in the T psi C loop of thermophile tRNA.Biochem Biophys Res Commun. 1979 Nov 28;91(2):671-7. doi: 10.1016/0006-291x(79)91574-2. Biochem Biophys Res Commun. 1979. PMID: 518660 No abstract available.
-
[The role of 2-thioribothymidine in the T psi C region of tRNA from an extreme thermophile on the thermal stability and the functional efficiency of the tRNA molecule (author's transl)].Tanpakushitsu Kakusan Koso. 1978 Oct;23(11):1106-22. Tanpakushitsu Kakusan Koso. 1978. PMID: 364538 Review. Japanese. No abstract available.
Cited by
-
Recognition of RNA duplexes by chemically modified triplex-forming oligonucleotides.Nucleic Acids Res. 2013 Jul;41(13):6664-73. doi: 10.1093/nar/gkt352. Epub 2013 May 8. Nucleic Acids Res. 2013. PMID: 23658228 Free PMC article.
-
Chemical and Conformational Diversity of Modified Nucleosides Affects tRNA Structure and Function.Biomolecules. 2017 Mar 16;7(1):29. doi: 10.3390/biom7010029. Biomolecules. 2017. PMID: 28300792 Free PMC article. Review.
-
Beyond the Anticodon: tRNA Core Modifications and Their Impact on Structure, Translation and Stress Adaptation.Genes (Basel). 2024 Mar 19;15(3):374. doi: 10.3390/genes15030374. Genes (Basel). 2024. PMID: 38540433 Free PMC article. Review.
-
Synthesis and studies on the effect of 2-thiouridine and 4-thiouridine on sugar conformation and RNA duplex stability.Nucleic Acids Res. 1997 Mar 15;25(6):1272-80. doi: 10.1093/nar/25.6.1272. Nucleic Acids Res. 1997. PMID: 9092639 Free PMC article.
-
Nucleoside modifications in the regulation of gene expression: focus on tRNA.Cell Mol Life Sci. 2016 Aug;73(16):3075-95. doi: 10.1007/s00018-016-2217-y. Epub 2016 Apr 19. Cell Mol Life Sci. 2016. PMID: 27094388 Free PMC article. Review.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous
