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. 1983 Jul;97(1):264-9.
doi: 10.1083/jcb.97.1.264.

A consistent picture of the actin filament related to the orientation of the actin molecule

W E Fowler, U Aebi

A consistent picture of the actin filament related to the orientation of the actin molecule

W E Fowler et al. J Cell Biol. 1983 Jul.

Abstract

We show that freeze-dried actin filaments which have been rotary shadowed with a light coat of platinum appear very similar in morphology and width to negatively-stained filaments. The addition of a thicker coat of platinum to such preparations gives the actin filaments a different morphology and width, which are similar to those of the rotary-shadowed, quick-frozen filaments described by Heuser and Kirschner (J. Cell Biol. 1980, 86:212-234). The consistent view of the actin filament presented here, particularly its 7-8-nm width, can be interpreted in terms of the overall orientation of the actin subunit in the actin filament.

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