Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1983 Jun 25;11(12):4229-39.
doi: 10.1093/nar/11.12.4229.

A general method to select for M13 clones carrying base pair substitution mutants constructed in vitro

C Traboni, R Cortese, G Ciliberto, G Cesareni
Free PMC article

A general method to select for M13 clones carrying base pair substitution mutants constructed in vitro

C Traboni et al. Nucleic Acids Res. .
Free PMC article

Abstract

In this paper we describe a method to select base pair substitution mutants constructed in vitro. The mutagenesis is performed by forcing mistakes during in vitro synthesis from a primer annealed to a single stranded DNA template. The selection is based on the fact that, following transformation, the progeny of the strand elongated in vitro and the template strand have different phenotypes. The method is general and applicable to any DNA segment; the type of base pair substitution and its position can be chosen at will. The combined efficiency of mutagenesis and selection allows for 85% frequency of mutants in all analyzed clones.

PubMed Disclaimer

References

    1. Virology. 1976 Mar;70(1):195-7 - PubMed
    1. Proc Natl Acad Sci U S A. 1977 Sep;74(9):3642-6 - PubMed
    1. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 - PubMed
    1. Nucleic Acids Res. 1978 Dec;5(12):4593-611 - PubMed
    1. Proc Natl Acad Sci U S A. 1980 Jul;77(7):4147-51 - PubMed

Publication types