Biochemistry and applications of aprotinin, the kallikrein inhibitor from bovine organs

Abstract

The basic proteinase inhibitor from bovine organs, aprotinin (active ingredient of Trasylol) has been extensively studied with respect to its chemical, physical and biochemical properties and its inhibitory mechanism of action. It is widely used as a valuable tool for studying protein/protein interactions and protein conformation at the molecular level. There are numerous examples of the usefulness of aprotinin in biochemical and biomedical research. It has also become a valuable drug for the treatment of various diseases like, e.g. hyperfibrinolytic hemorrhage and traumatic-hemorrhagic shock. The purpose of this paper is threefold. It summarizes our present knowledge of the subject in various disciplines; it provides the active scientist with basic data for his experimental work; and above all it points the way to future directions of aprotinin research.