Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1983 May;131(1):141-5.
doi: 10.1016/0003-2697(83)90145-8.

A rapid method for detecting specific RNA transcripts by hybridization to DNA probes in solution

F G Nobrega, C L Dieckmann, A Tzagoloff

A rapid method for detecting specific RNA transcripts by hybridization to DNA probes in solution

F G Nobrega et al. Anal Biochem. 1983 May.

Abstract

A method is described for detecting specific transcripts in crude mixtures of RNA. The method employs hybridization of single-stranded or double-stranded radioactive DNA probes in solution, followed by electrophoretic separation of the hybrid and probe on agarose and visualization by radioautography. The procedure offers the advantages of decreased preparation time and increased sensitivity over currently used methods.

PubMed Disclaimer

Publication types

LinkOut - more resources