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. 1983 May;131(1):18-24.
doi: 10.1016/0003-2697(83)90130-6.

Interaction between heparin and human pregnancy-associated plasma protein A (PAPP-A): a simple purification procedure

M W Davey, B Teisner, M Sinosich, J G Grudzinskas

Interaction between heparin and human pregnancy-associated plasma protein A (PAPP-A): a simple purification procedure

M W Davey et al. Anal Biochem. 1983 May.

Abstract

Human pregnancy-associated plasma protein A (PAPP-A) binds to heparin-Sepharose. This affinity chromatography preceded by molecular sieve chromatography provides a simple two-step purification procedure of PAPP-A from late pregnancy plasma. One hundred percent of the applied PAPP-A was recovered, with more than 40% being electrophoretically homogeneous after the two procedures. The remaining PAPP-A could be purified by negative affinity chromatography on anti-total human serum immobilized on agarose.

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