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. 1983 Jun;131(2):333-6.
doi: 10.1016/0003-2697(83)90178-1.

Detection of cellulase activity in polyacrylamide gels using Congo red-stained agar replicas

P Béguin

Detection of cellulase activity in polyacrylamide gels using Congo red-stained agar replicas

P Béguin. Anal Biochem. 1983 Jun.

Abstract

Bands that have cellulolytic activity are visualized after polyacrylamide gel electrophoresis by laying the slab gel on top of a thin sheet of 2% agar containing 0.1% carboxymethylcellulose. After a suitable incubation time, zones of carboxymethylcellulose hydrolysis are revealed by staining the agar replica with Congo red.

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