doi: 10.1016/0003-2697(83)90178-1.
Detection of cellulase activity in polyacrylamide gels using Congo red-stained agar replicas
- PMID: 6193735
- DOI: 10.1016/0003-2697(83)90178-1
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Detection of cellulase activity in polyacrylamide gels using Congo red-stained agar replicas
Anal Biochem.
1983 Jun.
Abstract
Bands that have cellulolytic activity are visualized after polyacrylamide gel electrophoresis by laying the slab gel on top of a thin sheet of 2% agar containing 0.1% carboxymethylcellulose. After a suitable incubation time, zones of carboxymethylcellulose hydrolysis are revealed by staining the agar replica with Congo red.
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