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. 1983;45(8):88-9.

[Modified method of staining semi-thin sections with hematoxylin and eosin]

[Article in Russian]
N S Migalkin, Iu M Ir'ianov
  • PMID: 6196005

[Modified method of staining semi-thin sections with hematoxylin and eosin]

[Article in Russian]
N S Migalkin et al. Arkh Patol. 1983.

Abstract

A modified method for staining of semi-thin sections with hematoxylin and eosin after removal of epoxide resin and osmium tetroxide from them is described. To enhance staining with hematoxylin, preliminary treatment with potassium permanganate and oxalic acid solutions was used. After staining with Harris hematoxylin differentiation and simultaneous toning of the nuclei with iron chloride were performed. Subsequent post-staining consists of a mixture of alcohol- and aqueous-soluble eosins acidified with glacial acetic acid.

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