doi: 10.1083/jcb.71.1.89.
Immunofluorescence evidence for the absence of histone H1 in a mitotically dividing, genetically inactive nucleus
- PMID: 61969
- PMCID: PMC2109720
- DOI: 10.1083/jcb.71.1.89
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Immunofluorescence evidence for the absence of histone H1 in a mitotically dividing, genetically inactive nucleus
J Cell Biol.
1976 Oct.
Abstract
Antibodies directed against whole histone and purified lysine-rich histone H1 extracted from isolated macronuclei of the ciliate Tetrahymena were obtained and conjugated to fluorescein isothiocyanate. The fluorescein-antibody conjugates were used to directly label Tetrahymena cells. Both macro- and micronuclei were visibly fluorescent in cells stained with anti-whole histone conjugate. However, the anti-H1 conjugate only labeled macronuclei. This in situ demonstration of the lack of positive immunofluorescent staining of micronuclei with anti-H1 conjugate provide further evidence for the absence of H1 in the genetically inactive, mitotically dividing Tetrahymena micronucleus.
