Comparison of machine and manual staining of direct smears for acid-fast bacilli by fluorescence microscopy
- PMID: 61973
- PMCID: PMC476217
- DOI: 10.1136/jcp.29.10.931
Comparison of machine and manual staining of direct smears for acid-fast bacilli by fluorescence microscopy
Abstract
Comparisons were made in Lusaka and in London between manual staining and staining in an automatic machine with auramine-phenol of direct smears of sputum and other types of specimen for acid-fast bacilli. No evidence was obtained of carry-over of acid-fast bacilli from positive to negative smears during machine staining. There was improved contrast between bacilli and the background in smears prepared with the machine.
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