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. 1983 Nov 11;95(1-2):131-3.
doi: 10.1016/0014-2999(83)90277-7.

Binding of [3H]substance P to putative substance P receptors in rat brain membranes

M H Perrone, R E Diehl, D R Haubrich

Binding of [3H]substance P to putative substance P receptors in rat brain membranes

M H Perrone et al. Eur J Pharmacol. .

Abstract

[3H]Substance P was used to label putative substance P receptors in rat brain membranes. [3H]Substance P binding was saturable, reversible, and displaceable by non-radioactive substance P. Association, dissociation and saturation experiments suggest a single site interaction of [3H]substance P to its binding site (k-1/k+1 = 0.56 nM; KD = 0.9 nM, Bmax 86.5 fmol/mg protein.

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