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. 1984 Mar 23;223(4642):1296-9.
doi: 10.1126/science.6199844.

Heparin affinity: purification of a tumor-derived capillary endothelial cell growth factor

Y Shing, J Folkman, R Sullivan, C Butterfield, J Murray, M Klagsbrun

Heparin affinity: purification of a tumor-derived capillary endothelial cell growth factor

Y Shing et al. Science. .

Abstract

A tumor-derived growth factor that stimulates the proliferation of capillary endothelial cells has a very strong affinity for heparin. This heparin affinity makes it possible to purify the growth factor to a single-band preparation in a rapid two-step procedure. The purified growth factor is a cationic polypeptide, has a molecular weight of about 18,000, and stimulates capillary endothelial cell proliferation at a concentration of about 1 nanogram per milliliter.

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