Myelin breakdown and elimination in the posterior funiculus of the adult cat after dorsal rhizotomy: a light and electron microscopic qualitative and quantitative study

Abstract

Adult cats were subjected to unilateral dorsal L6, L7, and S1 rhizotomy. After survival times of 1-1,552 days the degeneration of axons and myelin sheaths and the elimination of degenerating myelin was studied qualitatively and quantitatively with light and electron microscopy and in Marchi-stained sections in the posterior funiculus in T12-L2. Degeneration was first observed as swollen or shrunken nerve fibers. Somewhat later there was an increased occurrence of collapsed myelin sheaths. The latter lost their myelin periods and appeared to be transformed into myelin bodies. The occurrence of myelin bodies coincided temporally with the presence of many Marchi-positive bodies. Later, an increasing number of intracellularly located lipid droplets occurred, paralleled by the occurrence of a great number of Marchi-positive granules and crystalline structures. Profiles of collapsed myelin sheaths, myelin bodies, and lipid droplets were frequently seen in the cytoplasm of microglial cells. Later, astrocytes and perivascular cells became filled with numerous lipid droplets. The findings suggest that microglial cells take up collapsed myelin sheaths and within these cells the sheaths become transformed into myelin bodies and subsequently into lipid droplets. These two products of myelin disintegration appear to correspond to the Marchi-positive structures seen during the degeneration process. The lipid droplets appear to be transported to astrocytes and perivascular cells.