Culture of the terminally differentiated ventricular cardiac muscle cell. Characterization of exogenous substrate oxidation and the adenylate cyclase system

Abstract

Oxidation of several exogenous substrates by cultured adult rat ventricular cardiac muscle cells has been assessed. Unlike freshly isolated cardiac muscle cells which oxidize glucose preferentially, the cultured cells more closely resemble metabolically the in situ heart and the isolated perfused heart, in that their preference for exogenous substrates is in the order of fatty acid greater than glucose. This switch in metabolic preference from glucose to fatty acid is complete within 12 h after placing freshly isolated cells in culture. Glucose oxidation is stimulated by insulin and isoproterenol and inhibited by beta-hydroxybutyrate and octanoate. The adenylate cyclase system has also been examined in these cultured cells. Isoproterenol, norepinephrine and epinephrine stimulate the accumulation of cyclic adenosine 3':5'-monophosphate (cyclic AMP) in a concentration-dependent manner. The order of potency is isoproterenol greater than norepinephrine approximately equal to epinephrine. This stimulation is potentiated by 1-isobutyl-3-methylxanthine and inhibited by 1-propranolol.