doi: 10.1016/0167-4889(84)90105-8.
In vivo 19F-NMR of 5-fluorouracil incorporation into RNA and metabolites in Escherichia coli cells
- PMID: 6202324
- DOI: 10.1016/0167-4889(84)90105-8
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In vivo 19F-NMR of 5-fluorouracil incorporation into RNA and metabolites in Escherichia coli cells
Biochim Biophys Acta.
.
Abstract
19F resonances from RNA with 5-fluorouracil incorporated could be observed in intact Escherichia coli cells, as well as in tRNA isolated from the cells. 19F-NMR signals from the metabolic breakdown products of the fluorinated RNA were also detected in vivo. By observing the 19F-NMR spectrum, variations in the metabolic disposition of administered 5-fluorouracil could be monitored as a function of time and be compared when the cells were deprived of oxygen and other nutrients, subjected to ethidium bromide treatment, or grown in the presence of mitomycin C.
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