Escherichia coli O18ac antigen: structure of the O-specific polysaccharide moiety
- PMID: 6203837
- PMCID: PMC263301
- DOI: 10.1128/iai.45.1.203-209.1984
Escherichia coli O18ac antigen: structure of the O-specific polysaccharide moiety
Abstract
The O-specific polysaccharide moiety (O18ac polysaccharide) of the O18ac antigen (lipopolysaccharide) from Escherichia coli 2980 (O18ac:K5:Fim+:H-) was isolated in pure form by degradation of the lipopolysaccharide and chromatography on Sephadex G-50. The primary structure of the O18ac polysaccharide was elucidated by composition, fragmentation procedures, methylation analysis, and nuclear magnetic resonance spectroscopy. The polysaccharide consists of repeating units of the pentasaccharide: (formula; see text) which are joined in the polymer by alpha-1,2 linkages.
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