Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1984;194(3):357-61.
doi: 10.1007/BF00425544.

Induction of macrolide-lincosamide-streptogramin B resistance requires ribosomes able to bind inducer

Induction of macrolide-lincosamide-streptogramin B resistance requires ribosomes able to bind inducer

T J Gryczan et al. Mol Gen Genet. 1984.

Abstract

Plasmids were constructed containing the regulatory regions and N-terminal portions of ermC and of ermD , fused in phase with the coding sequence of the Escherichia coli lacZ gene. ermC and ermD are erythromycin (Em) inducible macrolide-lincosamide-streptogramin B resistance elements derived from Staphylococcus aureus and Bacillus licheniformis, respectively. The fusion plasmids were introduced into B. subtilis and used to study ermC and ermD regulation. In both cases, beta-galactosidase synthesis could be induced by low levels of Em. Induction was prevented by introduction of ole-2, a chromosomal mutation which decreases ribosomal affinity for Em. Induction also did not occur in the presence of intact copies of ermC , suggesting that prior or concomitant methylation of 23S rRNA, a treatment known to decrease ribosomal affinity for Em, was capable of interfering with ermC and ermD induction. These experiments are consistent with the translational attenuation model of ermC regulation, and together with other evidence, suggest that ermD is regulated by a similar mechanism.

PubMed Disclaimer

References

    1. Proc Natl Acad Sci U S A. 1980 Jul;77(7):3903-7 - PubMed
    1. J Bacteriol. 1971 Jun;106(3):835-47 - PubMed
    1. Nucleic Acids Res. 1981 Jun 11;9(11):2549-62 - PubMed
    1. Mol Gen Genet. 1981;182(2):341-8 - PubMed
    1. J Bacteriol. 1982 Nov;152(2):722-35 - PubMed

Publication types

LinkOut - more resources