Polymorphism and inheritance of serum esterases and beta-globulins in the paradise fish (Macropodus opercularis; Anabantidae)

Abstract

Electrophoretic variants of serum esterases and beta-globulins in two subspecies of paradise fish (Macropodus opercularis) were studied. Four esterase loci (Est-1, Est-2, Est-3 and Est-4), a single transferrin (Tf) and another major beta-globulin locus (Bg) were identified by segregational analysis. Est-3 seems to be a monomorphic locus. Three alleles of Est-1, two of Est-2, two of Est-4, four of Tf and two alleles of Bg were found in the laboratory population. None of these loci were closely linked. Electrophoretic patterns of F1 hybrids confirmed the monomeric structures of each of the studied proteins. Allelic segregation at the Tf and Bg loci was normal in F2 and backcross populations. In crosses of the two Macropodus subspecies there were deviations from Mendelian ratios because of missing recombinant esterase phenotypes. Each of these would have been homozygous Est-2f/f. We suppose that Est-2f/f causes lethality in the early phase of development, except in the Est-1c/c, Est-2f/f combination characteristic of the parental subspecies M.o. concolor.