Comparative Study
doi: 10.1016/0014-5793(84)81123-0.
Immunological identification of four different polypeptides in 'subunit VII' of mammalian cytochrome c oxidase
- PMID: 6204885
- DOI: 10.1016/0014-5793(84)81123-0
Item in Clipboard
Comparative Study
Immunological identification of four different polypeptides in 'subunit VII' of mammalian cytochrome c oxidase
FEBS Lett.
.
Free article
Abstract
Rat liver cytochrome c oxidase was separated by SDS-gel electrophoresis into 13 polypeptide bands. Monospecific antisera against the isolated polypeptides VIIa, VIIb and VIIc were raised in rabbits. Cytochrome c oxidase was blotted on nitrocellulose and incubated with the antisera. The antisera reacted only with their corresponding polypeptides, indicating no immunological relationship between polypeptides VIIa, VIIb and VIIc. The data also exclude that these polypeptides are proteolytic breakdown products of larger subunits.
Similar articles
-
Isolation and properties of cytochrome c oxidase from rat liver and quantification of immunological differences between isozymes from various rat tissues with subunit-specific antisera.Eur J Biochem. 1985 May 15;149(1):147-58. doi: 10.1111/j.1432-1033.1985.tb08905.x. Eur J Biochem. 1985. PMID: 2986969
-
Identification of tissue-specific isoforms for subunits Vb and VIIa of cytochrome c oxidase isolated from rainbow trout.Eur J Biochem. 1994 May 1;221(3):1111-6. doi: 10.1111/j.1432-1033.1994.tb18831.x. Eur J Biochem. 1994. PMID: 8181469
-
Structure of the cytochrome c oxidase complex of rat liver. 2. Topological orientation of polypeptides in the membrane as studied by proteolytic digestion and immunoblotting.Eur J Biochem. 1985 Jan 2;146(1):219-25. doi: 10.1111/j.1432-1033.1985.tb08641.x. Eur J Biochem. 1985. PMID: 2981684
-
The subunit composition and function of mammalian cytochrome c oxidase.Mitochondrion. 2015 Sep;24:64-76. doi: 10.1016/j.mito.2015.07.002. Epub 2015 Jul 17. Mitochondrion. 2015. PMID: 26190566 Review.
-
Isoforms of mammalian cytochrome c oxidase: correlation with human cytochrome c oxidase deficiency.Pediatr Res. 1990 Nov;28(5):529-35. doi: 10.1203/00006450-199011000-00024. Pediatr Res. 1990. PMID: 2175025 Review.
Cited by
-
Mitochondrial Protein Cox7b Is a Metabolic Sensor Driving Brain-Specific Metastasis of Human Breast Cancer Cells.Cancers (Basel). 2022 Sep 8;14(18):4371. doi: 10.3390/cancers14184371. Cancers (Basel). 2022. PMID: 36139533 Free PMC article.
-
Rapid evolution of the human gene for cytochrome c oxidase subunit IV.Proc Natl Acad Sci U S A. 1992 Jun 15;89(12):5266-70. doi: 10.1073/pnas.89.12.5266. Proc Natl Acad Sci U S A. 1992. PMID: 1319058 Free PMC article.
-
Isolation and characterization of human heart cytochrome c oxidase.J Bioenerg Biomembr. 1986 Aug;18(4):307-14. doi: 10.1007/BF00743051. J Bioenerg Biomembr. 1986. PMID: 3017929
-
The cytochrome c oxidase from the yeast Candida parapsilosis.J Bioenerg Biomembr. 1987 Oct;19(5):495-503. doi: 10.1007/BF00770032. J Bioenerg Biomembr. 1987. PMID: 2447069
-
A new procedure for the purification of monodisperse highly active cytochrome c oxidase from bovine heart.Biochem J. 1987 Mar 1;242(2):417-23. doi: 10.1042/bj2420417. Biochem J. 1987. PMID: 3036090 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
