Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1984 Jun;20(6):505-11.
doi: 10.1007/BF02619624.

Mouse hepatocyte synthesis and induction of the acute phase reactant: serum amyloid P-component

P T Le, R F Mortensen

Mouse hepatocyte synthesis and induction of the acute phase reactant: serum amyloid P-component

P T Le et al. In Vitro. 1984 Jun.

Abstract

A methodology for obtaining reproducible in vitro induction of the synthesis of the acute phase reactant serum amyloid P-component (SAP) by purified mouse hepatocytes was established. Optimal hepatocyte culture conditions for the induction and synthesis of SAP required certain hormones, a substratum for cell attachment, and activated macrophages. Leibowitz L15 medium had to be supplemented with dexamethasone, indomethacin, insulin, glucose, and fetal bovine serum. Purified mouse IL 1 could substitute for activated macrophages in the induction of SAP. Hepatocytes were allowed to adhere to a collagen matrix to enhance both cell viability and SAP synthesis induced by IL 1. Elicited macrophages cultured with hepatocytes were capable of augmenting SAP synthesis in the presence of IL 1.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Mol Immunol. 1980 May;17(5):571-7 - PubMed
    1. Proc Natl Acad Sci U S A. 1977 Feb;74(2):739-43 - PubMed
    1. Lab Invest. 1977 Apr;36(4):420-7 - PubMed
    1. In Vitro. 1981 Oct;17(10):913-25 - PubMed
    1. J Exp Med. 1978 Aug 1;148(2):466-77 - PubMed

Publication types

LinkOut - more resources