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. 1984 Aug 3;72(1):171-6.
doi: 10.1016/0022-1759(84)90445-9.

The effect of epitope density and antibody affinity on the ELISA as analysed by monoclonal antibodies

A M Lew

The effect of epitope density and antibody affinity on the ELISA as analysed by monoclonal antibodies

A M Lew. J Immunol Methods. .

Abstract

The antibody titre and the optical density values in an ELISA are influenced by the epitope density of the antigen and the affinity of the antibody tested. This has major implications in the interpretation of ELISA results.

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