5-Azacytidine-induced undercondensations in human chromosomes
- PMID: 6205969
- DOI: 10.1007/BF00291352
5-Azacytidine-induced undercondensations in human chromosomes
Abstract
The cytosine analogue 5-azacytidine induces very distinct undercondensations in human chromosomes if applied to lymphocyte cultures. The number of induced undercondensations and their chromosomal localization can be varied by the 5-azacytidine dose and the treatment time. "Pulverized" chromosomes or undercondensations in the G-band-positive chromosome regions are produced with high doses and long treatment times. If applied in low doses during the last hours of culture, 5-azacytidine induces specific undercondensations in the heterochromatin of chromosomes 1, 9, 15, 16, and Y. Optimum conditions required for inducing the various types of undercondensation in the chromosomes were determined. Various examples of the use of 5-azacytidine in the analysis of chromosome rearrangements involving heterochromatic regions are presented.
Similar articles
-
A direct demonstration of somatically paired heterochromatin of human chromosomes.Cytogenet Cell Genet. 1983;36(3):554-61. doi: 10.1159/000131972. Cytogenet Cell Genet. 1983. PMID: 6196158
-
Distamycin A/DAPI bands and the effects of 5-azacytidine on the chromosomes of the chimpanzee, Pan troglodytes.Cytogenet Cell Genet. 1984;38(3):192-9. doi: 10.1159/000132059. Cytogenet Cell Genet. 1984. PMID: 6207990
-
Heterochromatin in the chromosomes of the gorilla: characterization with distamycin A/DAPI, D287/170, chromomycin A3, quinacrine, and 5-azacytidine.Cytogenet Cell Genet. 1986;41(2):71-82. doi: 10.1159/000132207. Cytogenet Cell Genet. 1986. PMID: 2420535
-
The effects of 5-azacytidine and 5-azadeoxycytidine on chromosome structure and function: implications for methylation-associated cellular processes.Pharmacol Ther. 1995 Jan;65(1):19-46. doi: 10.1016/0163-7258(94)00053-6. Pharmacol Ther. 1995. PMID: 7536332 Review.
-
Heterochromatin polymorphism and human cancer.Cancer Genet Cytogenet. 1981 Apr;3(3):261-72. doi: 10.1016/0165-4608(81)90093-5. Cancer Genet Cytogenet. 1981. PMID: 7026025 Review. No abstract available.
Cited by
-
Preferential somatic pairing between homologous heterochromatic regions of human chromosomes.Am J Hum Genet. 1986 Mar;38(3):319-29. Am J Hum Genet. 1986. PMID: 2420175 Free PMC article.
-
Trichostatin A and 5-azacytidine both cause an increase in global histone H4 acetylation and a decrease in global DNA and H3K9 methylation during mitosis in maize.BMC Plant Biol. 2010 Aug 18;10:178. doi: 10.1186/1471-2229-10-178. BMC Plant Biol. 2010. PMID: 20718950 Free PMC article.
-
An extra band within the human 9qh+ region that behaves like the surrounding constitutive heterochromatin.J Med Genet. 1994 Aug;31(8):632-4. doi: 10.1136/jmg.31.8.632. J Med Genet. 1994. PMID: 7529317 Free PMC article.
-
Rare variants of chromosome 9 with extra G positive band within the qh region are not alike.J Med Genet. 1995 May;32(5):405-6. doi: 10.1136/jmg.32.5.405. J Med Genet. 1995. PMID: 7542346 Free PMC article. No abstract available.
-
2'-Deoxyriboguanylurea, the primary breakdown product of 5-aza-2'-deoxyribocytidine, is a mutagen, an epimutagen, an inhibitor of DNA methyltransferases and an inducer of 5-azacytidine-type fragile sites.Nucleic Acids Res. 2012 Oct;40(19):9788-801. doi: 10.1093/nar/gks706. Epub 2012 Jul 31. Nucleic Acids Res. 2012. PMID: 22850746 Free PMC article.
References
MeSH terms
Substances
LinkOut - more resources
Research Materials