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Comparative Study
. 1984 Jun;19(6):937-9.
doi: 10.1128/jcm.19.6.937-939.1984.

Indirect immunofluorescence staining of Chlamydia trachomatis inclusions in microculture plates with monoclonal antibodies

M Zapata, M Chernesky, J Mahony
Comparative Study

Indirect immunofluorescence staining of Chlamydia trachomatis inclusions in microculture plates with monoclonal antibodies

M Zapata et al. J Clin Microbiol. 1984 Jun.

Abstract

Indirect immunofluorescence (IF) staining, using a monoclonal antibody, detected two- to fourfold more inclusions than did iodine staining. Of 274 clinical specimens, 53 (19.3%) were positive by IF on passage 1 as compared with 33 (12%) by iodine staining (P less than 0.005). IF-stained inclusions in McCoy cells in the bottom of microculture wells were readily viewed with a long-focal-length objective at a magnification of 250 X.

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References

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