A silver method for counterstaining plastic embedded tissue
- PMID: 6206616
- DOI: 10.3109/10520298309066814
A silver method for counterstaining plastic embedded tissue
Abstract
This report presents a method which can be used for counterstaining semithin sections of plastic embedded tissue. The sections are treated with a solution of silver lactate, followed by physical development. During the silver lactate treatment, silver ions are bound by various tissue components as metallic silver or silver sulfide. During physical development catalytic reduction of silver ions to metallic silver takes place where silver has been bound in the tissue, enlarging the silver deposits to microscopically visible dimensions. The amplified silver deposits give high contrast staining in yellow, brown and black suitable for both color and monochrome photography. The localization of the silver deposits is highly specific and may reflect several independent chemical processes. Examples in several tissues are shown.
Similar articles
-
Silver amplification of mercury sulfide and selenide: a histochemical method for light and electron microscopic localization of mercury in tissue.J Histochem Cytochem. 1985 Mar;33(3):219-28. doi: 10.1177/33.3.2579122. J Histochem Cytochem. 1985. PMID: 2579122
-
Polychromatic staining of epoxy semithin sections: a new and simple method.Histochemistry. 1994 Jan;101(1):51-5. doi: 10.1007/BF00315831. Histochemistry. 1994. PMID: 7517926
-
Application of Grimelius argyrophil staining to the study of tumour ultrastructure. III. Effects of osmium tetroxide.Acta Histochem. 1989;86(1):1-14. doi: 10.1016/S0065-1281(89)80039-X. Acta Histochem. 1989. PMID: 2475997
-
Argentaffin staining of elastic material in semithin sections.Mikroskopie. 1982 Oct;39(9-10):263-71. Mikroskopie. 1982. PMID: 6183615 No abstract available.
-
[Hematoxylin and eosin staining of semi-thin sections of aldehyde-osmium tetroxide-fixed tissues embedded in epoxy resin "Quetol 812"].Kaibogaku Zasshi. 1984 Feb;59(1):3-8. Kaibogaku Zasshi. 1984. PMID: 6206679 Japanese. No abstract available.
Cited by
-
The autometallographic zinc-sulphide method. A new approach involving in vivo creation of nanometer-sized zinc sulphide crystal lattices in zinc-enriched synaptic and secretory vesicles.Histochem J. 1996 May;28(5):361-73. doi: 10.1007/BF02331399. Histochem J. 1996. PMID: 8818683
-
Light microscopical detection of leukocyte cell surface antigens with a one-nanometer gold probe.Histochemistry. 1991;95(5):483-90. doi: 10.1007/BF00315744. Histochemistry. 1991. PMID: 1714436
-
Quantification of vesicular zinc in the rat brain.Histochemistry. 1988;89(3):289-93. doi: 10.1007/BF00493154. Histochemistry. 1988. PMID: 2457004
-
Autometallography: tissue metals demonstrated by a silver enhancement kit.Histochemistry. 1987;86(5):465-9. doi: 10.1007/BF00500618. Histochemistry. 1987. PMID: 3583820
-
Histochemical differentiation of autometallographically traceable metals (Au, Ag, Hg, Bi, Zn): protocols for chemical removal of separate autometallographic metal clusters in Epon sections.Histochem J. 2000 Nov;32(11):645-52. doi: 10.1023/a:1004115130843. Histochem J. 2000. PMID: 11272803