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. 1984 Oct;52(1):290-2.
doi: 10.1128/JVI.52.1.290-292.1984.

Monoclonal antibodies to a monkeypox virus polypeptide determinant

L F Roumillat, J L Patton, M L Davis

Monoclonal antibodies to a monkeypox virus polypeptide determinant

L F Roumillat et al. J Virol. 1984 Oct.

Abstract

Three monkeypox virus (MPV) antibody-secreting murine monoclones were characterized as being of the immunoglobulin G1 isotype, gave a 4+ reaction in the indirect fluorescent-antibody test, gave a positive reaction in the enzyme immunoassay, and did not neutralize MPV. These monoclonal antibodies were determined by the sodium dodecyl sulfate-polyacrylamide gel electrophoresis transblot method to react to a 15,500-molecular-weight MPV polypeptide. This reactivity could not be removed by adsorption to a vaccinia virus-infected cell suspension. The three monoclonal antibodies were specific for MPV when tested against epidemiologically unrelated isolates of cowpox virus, variola virus, vaccinia virus, and MPV.

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