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. 1984 Sep 25;12(18):7057-70.
doi: 10.1093/nar/12.18.7057.

Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs

P A Krieg, D A Melton
Free PMC article

Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs

P A Krieg et al. Nucleic Acids Res. .
Free PMC article

Abstract

We describe a method for the synthesis of microgram quantities of eucaryotic messenger RNAs. Injection into the cytoplasm of frog oocytes and addition to wheat germ extracts show that these synthetic RNAs function efficiently as messenger RNAs. We confirm that a 5' cap on the mRNA is essential for translation in injected oocytes and show that most of the 3' flanking region, including the poly A tail, can be deleted without the abolition of protein synthesis. The method of mRNA synthesis involves in vitro transcription of cDNAs which have been cloned into SP6 vectors (described in the accompanying paper). This method enables one to produce large amounts of mRNA and consequently protein from any cDNA clone.

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References

    1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
    1. Nature. 1971 Sep 17;233(5316):177-82 - PubMed
    1. J Mol Biol. 1973 Nov 5;80(3):539-51 - PubMed
    1. Proc Natl Acad Sci U S A. 1974 Aug;71(8):3143-6 - PubMed
    1. Cell. 1974 Jul;2(3):189-96 - PubMed

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