doi: 10.1007/BF00704063.
Immunoelectron microscopy of amylase in the human parotid gland. Ultrastructural localization by use of both the protein A-gold and the biotin-avidin-gold technique
- PMID: 6207660
- DOI: 10.1007/BF00704063
Item in Clipboard
Immunoelectron microscopy of amylase in the human parotid gland. Ultrastructural localization by use of both the protein A-gold and the biotin-avidin-gold technique
Virchows Arch A Pathol Anat Histopathol.
1984.
Abstract
Specimens of the human parotid gland were studied by immuno-electron microscopy for the presence of amylase. Both the protein A-gold technique and the biotin-avidin-gold technique were used on the same specimens. Different fixations were tried. Amylase was detected in the zymogen granules in high amounts. This enzyme could even be seen in glutaraldehyde fixed and routinely embedded material. The subcellular localization of this enzyme opens a new field of functional morphological studies and studies in special tumours including acinic cell carcinomas.
Similar articles
-
Immunocytochemical localization of amylase in the parotid gland of developing and adult rats.J Histochem Cytochem. 1981 Oct;29(10):1189-95. doi: 10.1177/29.10.6170667. J Histochem Cytochem. 1981. PMID: 6170667
-
Rapid primary microwave-aldehyde and microwave-osmium fixation: improved detection of rat parotid acinar cell secretory granule alpha-amylase using a post-embedding immunogold ultrastructural morphometric analysis.J Histochem Cytochem. 1995 May;43(5):515-23. doi: 10.1177/43.5.7730590. J Histochem Cytochem. 1995. PMID: 7730590
-
Effects of valinomycin on osmotic lysis of zymogen granules and amylase exocytosis from parotid acini.Am J Physiol. 1993 May;264(5 Pt 1):G895-901. doi: 10.1152/ajpgi.1993.264.5.G895. Am J Physiol. 1993. PMID: 7684567
-
Liquid-diet-induced alterations of rat parotid acinar cells studied by electron microscopy and enzyme cytochemistry.Arch Oral Biol. 1981;26(5):369-80. doi: 10.1016/0003-9969(81)90032-7. Arch Oral Biol. 1981. PMID: 6172099 No abstract available.
-
Morphological and histochemical changes in the secretory granules of mucous cells in the early postnatal mouse parotid gland.Arch Histol Cytol. 2001 Aug;64(3):259-66. doi: 10.1679/aohc.64.259. Arch Histol Cytol. 2001. PMID: 11575422
Cited by
-
Binding sites of Ulex europaeus-lectin I in human parotid gland. A light-microscopic and ultrastructural study using the immunoperoxidase technique and immunocryoultramicrotomy.Cell Tissue Res. 1987 May;248(2):455-61. doi: 10.1007/BF00218213. Cell Tissue Res. 1987. PMID: 3555837
-
Subcellular localization of epidermal growth factor in human parotid gland.Histochem J. 2001 Jul;33(7):427-31. doi: 10.1023/a:1013780028887. Histochem J. 2001. PMID: 11858463
-
On approaches to the functional restoration of salivary glands damaged by radiation therapy for head and neck cancer, with a review of related aspects of salivary gland morphology and development.Biotech Histochem. 2008 Jun;83(3-4):103-30. doi: 10.1080/10520290802374683. Biotech Histochem. 2008. PMID: 18828044 Free PMC article. Review.