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. 1984 Nov;81(21):6594-8.
doi: 10.1073/pnas.81.21.6594.

Expression of human alpha 1-antitrypsin cDNA in the yeast Saccharomyces cerevisiae

T Cabezón, M De Wilde, P Herion, R Loriau, A Bollen

Expression of human alpha 1-antitrypsin cDNA in the yeast Saccharomyces cerevisiae

T Cabezón et al. Proc Natl Acad Sci U S A. 1984 Nov.

Abstract

Nucleotide sequences coding either for the precursor or the mature form of human alpha 1-antitrypsin have been placed under the control of the yeast ARG3 expression signals. Recombinant plasmids pRIT10782 and pRIT10787 express the precursor or the mature alpha 1-antitrypsin species, respectively, in two different yeast strains, with yields ranging between 0.3 and 1% of total soluble proteins. The alpha 1-antitrypsin synthesized in yeasts was specifically recognized by polyclonal and monoclonal antibodies raised against human alpha 1-antitrypsin. In addition, it was shown to be biologically active in its mature form only, with optimal activity in a peptidase-deficient yeast strain.

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References

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