Oxidized p-phenylenediamine staining of epoxy resin sections
- PMID: 6208701
- DOI: 10.1515/znc-1984-7-824
Oxidized p-phenylenediamine staining of epoxy resin sections
Abstract
Semithin and thin sections of glutaraldehyde-fixed, epoxy resin-embedded animal tissues were treated with solutions of oxidized p-phenylenediamine (PPD). This method is suitable to reveal the general morphology of tissues in light microscopy, showing a high staining degree in some polyanion containing components. Posttreatments of thin sections with gold chloride solutions give considerable electron opacity in PPD positive structures.