In situ assay of poxvirus-induced and virion-contained deoxyribonucleases in DNA polyacrylamide gels
- PMID: 621135
- DOI: 10.1159/000148915
In situ assay of poxvirus-induced and virion-contained deoxyribonucleases in DNA polyacrylamide gels
Abstract
Cowpox virus and vaccinia virus WR specific DNases were identified by an in situ assay using DNA-containing acrylamide gels. A DNase present in the isolated virions of strain cowpox and vaccinia WR was identified. Its properties as determined by the in situ assay resembled those of the well-characterized DNase V1. The assay was also used to follow the time course of 'acid' DNase induction by cowpox virus in primary chick embryo fibroblasts.
Similar articles
-
Synthesis of early vaccinia-virus-specific enzymes under conditions of immediate early gene expression.Eur J Biochem. 1984 Sep 17;143(3):589-91. doi: 10.1111/j.1432-1033.1984.tb08410.x. Eur J Biochem. 1984. PMID: 6479164
-
Studies on the polypeptides of poxvirus. I. Comparison of structural polypeptides in vaccina, cowpox and Shope fibroma viruses.Biken J. 1978 Jun;21(2):51-61. Biken J. 1978. PMID: 219840
-
Isolation of early viral proteins from poxvirus-infected chick embryo fibroblasts by DNA-cellulose chromatography and inhibition of their synthesis by chicken interferon.Eur J Biochem. 1983 May 2;132(2):361-7. doi: 10.1111/j.1432-1033.1983.tb07371.x. Eur J Biochem. 1983. PMID: 6188613
-
Purification of mRNA guanylyltransferase from vaccinia virions.J Biol Chem. 1978 Jun 25;253(12):4481-9. J Biol Chem. 1978. PMID: 659428
-
In a nutshell: structure and assembly of the vaccinia virion.Adv Virus Res. 2006;66:31-124. doi: 10.1016/S0065-3527(06)66002-8. Adv Virus Res. 2006. PMID: 16877059 Review.
Cited by
-
Isoelectric focusing--polynucleotide/polyacrylamide-gel electrophoresis. A technique to separate and characterize nuclease activities.Biochem J. 1984 Apr 15;219(2):553-61. doi: 10.1042/bj2190553. Biochem J. 1984. PMID: 6743232 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources